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After TEV digestion overnight at 4 °C, the elution was then subjected to Strep-Tactin Sepharose resin (IBA, 2-1201-010), washed with wash buffer and eluted with wash buffer supplemented with 10 mM desthiobiotin (Sigma-Aldrich, D1411)

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doi: 10.1016/0041-0101(93)90137-8 106 CoelhoFMPessiniACCoelhoAMPinhoVSSouzaDGArantesECet al